目的 采用激光显微共聚焦拉曼光谱仪研究卡马西平片晶型并建立定性判别模型。方法 建立卡马西平 4 种晶型的拉曼对照图谱，用OPUS 软件聚类分析法判别出国内不同厂家卡马西平片的晶型，再通过建立定性鉴别模型鉴别未知样品。结果 聚类分析结果表明，4批卡马西平片均属于晶型Ⅲ，采用一阶导数法和矢量归一化法处理图谱，用因子分析法计算，选择200~500、650~850、1 000~1 100、1 500~1 700 cm-1谱段建立定性模型，经验证，该模型的灵敏度、专属性、阴性预报性和检出效率均良好。结论 激光显微共聚焦拉曼光谱仪可用于卡马西平片的晶型判别及实现快速、准确的定性鉴别。
Abstract
OBJECTIVE To analyze the polymorphs of carbamazepin tablets by laser confocal micro-Raman spectrometer and establish the identification test model. METHODS Reference Raman spectra of the four crystal forms of carbamazepin were established; then the polymorphs of the carbamazepin tablets from different manufacturers were analyzed,and an identification test model was established to distinguish the unknown samples. RESULTS According to the results of the cluster analysis,the four batches of carbamazepin tablets from domestic manufacturers belonged to crystal formⅢ. A qualitative model was created by choosing the first derivative and vector normalization method to process the spectra,using the factorization method to calculate,and selecting the 200-500, 650-850, 1 000-1 100, 1 500-1 700 cm-1 regions to compare. This model was verified to have high sensitivity, specificity, precisicion, negative prediction ability, and detection efficiency. CONCLUSION The laser confocal micro-Raman spectrometer canbe used to analyze the polymorphs of carbamazepin tablets and the qualitative identification is rapid and accurate.
关键词
激光显微共聚焦拉曼光谱 /
卡马西平 /
晶型判别 /
定性鉴别模型 /
聚类分析 /
因子分析法
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Key words
laser confocal micro-Raman spectra /
carbamazepin tablet /
polymorphs analysi /
identificationtest model /
cluster analysis /
factorization method
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中图分类号：
R917
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参考文献
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脚注
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基金
浙江省分析测试科技计划资助项目(2015C37056)
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